In many molecular mechanisms, the three-dimensional structure of proteins allows to explain fundamental processes such as the binding of partners, catalysis or allostery. At the end of the course, the student should be able to analyse a three-dimensional (3D) structure of proteins, to have a critical point of view about the validity of the proposed structure, and to familiarized with the techniques of 3D structure determination and prediction.
Familiarize the student with tridimensional (3D) structure of proteins, from experimental determination until prediction methods. Protein engineering will also be discussed, with examples from the scientific literature and invited speakers.
Experimental and theoretical approaches linked to the modeling of proteins 3D structures. Limits and possibilities offered by protein engineering.
- Basic introduction about proteins 3D structures
- 3D structure determination of proteins (XRD, NMR) : concepts, applications and examples
- 3D structure prediction
- Conformational changes
- Function prediction for proteins
- 3D structure of membrane proteins
- Protein engineering
A practical course with the cristallisation of a protein and initial analysis by X ray diffraction is proposed
The course is given with powerpoint presentations that are available in PDF format from the Webcampus website. Invited speakers active in the field of protein structure and function are invited to present their research to the students.
The evaluation method will be explained during the first course. The evaluation will take the form of an interview, where two topics will be addressed:
a discussion about a publication that will be available in advance on Webcampus
a discussion on the topic addressed by one of the external guests
A PDF version of the slides is available on the Webcampus site.
| Training | Study programme | Block | Credits | Mandatory |
|---|---|---|---|---|
| Master in Physics | Standard | 1 | 3 | No |
| Master in Biology | Standard | 1 | 3 | No |
| Master in Physics | Finalité didactique | 1 | 3 | No |
| Master in Biomedical Sciences | Standard | 1 | 3 | No |
| Master in Biomedical Sciences | Filière en recherche fondamentale et préclinique | 1 | 3 | No |
| Master in Biomedical Sciences | Filière en gestion de la recherche clinique | 1 | 3 | No |
| Master in Physics | Finalité spécialisée en physique et data | 1 | 3 | No |
| Master in Biochemistry and Molecular and Cell Biology | Finalité approfondie | 1 | 3 | No |
| Master in Biochemistry and Molecular and Cell Biology | Finalité spécialisée | 1 | 3 | No |
| Master in Physics | Finalité spécialisée en physique du vivant | 1 | 3 | No |
| Master in Physics | Finalité approfondie | 1 | 3 | No |
| Master in Physics | Finalité approfondie | 2 | 3 | No |
| Master in Physics | Finalité didactique | 2 | 3 | No |
| Master in Physics | Finalité spécialisée en physique et data | 2 | 3 | No |
| Master in Physics | Finalité spécialisée en physique du vivant | 2 | 3 | No |