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The program 09:30 | Welcome speech and coffee10:00 | Presentation of the platforms11:00 | Group visit of the platforms12:00 | Lunch and networking Pysico-Chemical Characterization (PC²)The PC² platform comprises a wide range of instruments, including: liquid and solid-state nuclear magnetic resonance spectrometers, X-ray diffractometers for single crystals and powders, instruments for analyzing textural properties (nitrogen physisorption, mercury porosimetry, etc.), instruments for analyzing chemical composition (combustion chemical analysis, ICP-OES, etc.), as well as various separation techniques (chromatography, centrifugation, etc.).), instruments for analyzing chemical composition (combustion chemical analysis, ICP-OES, etc.), and various separation techniques (chromatography, centrifugation, etc.). The combination of these techniques with the presence of two research logisticians and a technician dedicated to sample analysis, as well as highly qualified researchers for the development of advanced applications, reflects the strategic intent of this platform. Among these characterization techniques, solid-state NMR and X-ray diffraction are the most advanced and unique characterization tools.Synthesis, Irradiation and Analysis of Materials (SIAM)The SIAM platform specializes in the advanced synthesis and characterization of materials and nanomaterials. It actively contributes to fundamental research in (bio)materials science, particularly in terms of characterizing surfaces, interfaces and ion/material interactions, in collaboration with international university laboratories. SIAM's analytical capabilities enable it to study a wide range of samples from fields as diverse as materials science, life sciences and heritage science. One of SIAM's key assets is its recognized expertise in spectroscopy (XPS and ToF-SIMS), which can be coupled with nuclear analysis (Ion Beam Analysis or IBA). Thanks to state-of-the-art equipment, all support is provided by a highly qualified team in a dynamic of continuous development and innovation. As part of the University of Namur, SIAM is a privileged partner both for academic research projects and for the provision of services to industrial and institutional players.Lasers, Optics and Spectroscopies (LOS)The LOS platform is developing its expertise around optical methods for the study of materials. LOS recently acquired a Raman scattering microscope for the analysis of liquids, powders, solids and thin films, both organic and inorganic. This technique can be used to identify a sample's chemical composition and structure, as well as certain properties of the medium. Raman spectroscopy can be used to characterize polymers, nanomaterials, pharmacological compounds, geological materials, precious stones, heritage objects and food products, to name but a few. In imaging mode, this technique can map the distribution of a compound in a heterogeneous sample, as well as detect traces. Practical information Registration required before November 4, 2025. I want to register Find out more about UNamur's technology platforms Contact Research Administration | Business Developer - Joël Marinozzi : joel.marinozzi@unamur.be

Abstract Primarily described as an alarmone, secondary messenger (p)ppGpp, when accumulated, binds to many targets involved in DNA replication, translation, and transcription. In the asymmetrically-dividing a-proteobacterium Caulobacter crescentus, (p)ppGpp has been shown to strongly impact cell cycle progression and differentiation, promoting the non-replicating G1/swarmer phase. Mutations in the major subunits of transcriptional complex, b or b' subunits, were able to display the (p)ppGpp-related phenotypes even in the absence of the alarmone. We identified that the transcriptional holo-enzyme, RNA polymerase (RNAP) is a primary target of (p)ppGpp in C. crescentus. Furthermore, mutations that inactivate (p)ppGpp binding to RNAP annihilated the (p)ppGpp-related phenotypes and phenocopied a (p)ppGpp0 strain. Our RNAseq analysis further elucidated the changes in the transcriptional landscape of C. crescentus cells displaying different (p)ppGpp levels and expressing RNAP mutants. Since the DNA replication initiation protein DnaA is required to exit the G1 phase, we observed that it was significantly less abundant in cells accumulating (p)ppGpp. We further explored its proteolysis under the influence of (p)ppGpp. Our work suggests that (p)ppGpp regulates cell cycle and differentiation in C. crescentus by reprogramming transcription and triggering proteolytic degradation of key cell cycle regulators by yet unknown mechanisms. In Part II, we identified two σ factors belonging to the ECF family that might be involved in this (p)ppGpp-accompanied phenotypes. In Part III, we propose an overlapping role of the ω subunit, RpoZ, and the heat shock subunit, RpoH, in carbon metabolism.JuryProf. Gipsi LIMA MENDEZ (UNamur), PresidentProf Régis HALLEZ (UNamur), SecretaryDr Emanuele BIONDI (CNRS-Université Paris-Saclay)Prof. Justine COLLIER (University of Lausanne)Dr Marie DELABY (Université de Montréal)

Abstract Estrogens originating from human and animal excretion, as well as from anthropogenic sources such as cosmetics, plastics, pesticides, detergents, and pharmaceuticals, are among the most concerning endocrine-disrupting compounds in aquatic environments due to their potent estrogenic activity. While their effects on fish reproduction are well documented, their impact on development, particularly metamorphosis, remains poorly studied. This hormonal transition, mainly controlled by the thyroid axis, is essential for the shift from the larval to the juvenile stage in teleosts.The effects of two contraceptive estrogens on zebrafish (Danio rerio) metamorphosis were evaluated: 17α-ethinylestradiol (EE2), a synthetic reference estrogen, and estetrol (E4), a natural estrogen recently introduced in a new combined oral contraceptive formulation. Continuous exposure from fertilization to the end of metamorphosis allowed the assessment of morphological changes, disruptions of the thyroid axis, and modifications of additional molecular pathways potentially involved in metamorphic regulation.EE2 induced significant delays and disturbances in metamorphosis, affecting both internal and external morphological traits, confirming its role as an endocrine disruptor of concern. In contrast, E4 did not cause any detectable morphological alterations even at concentrations far exceeding those expected in the environment, indicating a limited ecotoxicological risk. Molecular analyses showed that EE2 strongly affected thyroid signaling and energy metabolism during metamorphosis, whereas E4 induced only minor transcriptional and proteomic changes.This study provides the first evidence that EE2 can disrupt zebrafish metamorphosis and highlights the importance of including this developmental stage in ecotoxicological assessments. The results also suggest a larger environmental safety margin for E4, although further research is needed to clarify the mechanisms linking estrogen exposure to metamorphic regulation.JuryProf. Frederik DE LAENDER (UNamur), PresidentProf. Patrick KESTEMONT (UNamur), SecretaryDr. Sébastien BAEKELANDT (UNamur)Dr. Valérie CORNET (UNamur)Prof. Jean-Baptiste FINI (Muséum National d'Histoire Naturelle, Paris)Dr. Marc MULLER (ULiège)Prof. Veerle DARRAS (KULeuven)

In early September, the University of Namur hosted the first Main-Group Elements Reactivity Conference (MG-ERC). Over 100 researchers from 12 countries and 32 institutions gathered around Professor Guillaume Berionni. An event hailed as "one of the best chemistry conferences" by its prestigious guests.