Learning outcomes

Theoretical and practical approach of standard methods in cell and tissue biology in order to identify their field of application, usefulness and limitations.

Goals

To understand, perform and describe basic experiments in preparation for the master thesis. Therefore, access is restricted to students in their final bachelor year.

Content

  • Microscopy
  • Tissue staining, histochemistry, immunohistochemistry
  • Extraction, purification and analysis of RNA
  • Analysis of proteins
  • Reporter systems and tags
  • Cell culture
  • Transfection of cultured mammalian cells

 

Table of contents

  • Microscopy
  • Tissue staining, histochemistry, immunohistochemistry
  • Extraction, purification and analysis of RNA
  • Analysis of proteins
  • Reporter systems and tags
  • Cell culture
  • Transfection of cultured mammalian cells

 

Exercices

Students achieve a practical approach of several basic methods and learn how to write a lab report.

Assessment method

 
The theoretical examination is oral or written. Assessment procedures are subject to change in the event of force majeure.
 
Practical work reports are assessed according to criteria specified by the assistant in charge. The weighting of theory and practical work in the final grade for the teaching unit (UE) is 60/40.
 
If a mark of less than 10 is awarded for either the practical work or the theory exam, the final grade for the UE will be set at the lower mark. In the event of a single failure in the theory exam, only the theory part will be taken at the August session. In the event of a single failure in the practical part, an oral or written examination on the practical part must be taken at the August session.

Sources, references and any support material

Molecular cloning : A laboratory manual. Green and sambrook, 4th edition, Cold Spring Harbor Laboratory Press 2012.

Culture of animal cells : A manual of basic techniques and specialized applications. R.Ian Freshney, 7th edition, Wiley and sons, Hoboken New Jersey 2016

Language of instruction

French
Training Study programme Block Credits Mandatory
Bachelor in Biomedical Sciences Standard 0 4
Bachelor in Biomedical Sciences Standard 3 4