Learning outcomes

At the end of this theoretical and practical course, students will be able to understand, carry out and describe fundamental laboratory manipulations with a view to their Master's studies (thesis). This teaching unit is therefore reserved for students at the end of their bachelor cycle.

Goals

The aim of this course is to provide a theoretical and practical introduction to the basic methods used to study cells and tissues, in order to identify their fields of application, their advantages and their limitations.

Content

  • Basic microscopy techniques
  • Tissue staining, histochemistry, immunohistochemistry
  • Extraction, purification and analysis of RNA
  • Analysis of proteins
  • Reporter systems and tags
  • Cell culture
  • Transfection of cultured mammalian cells

 

Table of contents

  • Preparation of cells ans tissues for microscopy
  • Tissue staining, histochemistry, immunohistochemistry
  • Principles of electron microscopy
  • Extraction, purification and analysis of RNA
  • Analysis of proteins
  • Reporter systems and tags
  • Cell culture
  • Transfection of cultured mammalian cells

 

Exercices

Students achieve a practical approach of several basic methods and learn how to write a lab report.

Teaching methods

The different methods are discussed in theory, to explain their basic principles and limitations. PowerPoint support and course notes are available.

Assessment method

 

The theoretical examination is oral or written. Assessment procedures are subject to change in the event of force majeure.

 

Practical work reports are assessed according to criteria specified by the assistant in charge. The weighting of theory and practical work in the final grade for the teaching unit (UE) is 60/40.

 

If a mark of less than 10 is awarded for either the practical work or the theory exam, the final grade for the UE will be set at the lower mark. In the event of a single failure in the theory exam, only the theory part will be taken at the August session. In the event of a single failure in the practical part, an oral or written examination on the practical part must be taken at the August session.

A TP grade higher than 10/20 may be carried forward to a subsequent academic year.

Sources, references and any support material

Molecular cloning : A laboratory manual. Green and sambrook, 4th edition, Cold Spring Harbor Laboratory Press 2012.

Culture of animal cells : A manual of basic techniques and specialized applications. R.Ian Freshney, 7th edition, Wiley and sons, Hoboken New Jersey 2016

Language of instruction

French
Training Study programme Block Credits Mandatory
Bachelor in Biomedical Sciences Standard 0 4
Bachelor in Biomedical Sciences Standard 3 4