We analyze your cells, regardless of the organism, tissue, or cell culture, using flow cytometry. Our experts support you every step of the way: experimental design, data acquisition, instrument training, and analysis. On this page, discover our applications, our expertise, our equipment, our services, and our prices.
Cell analysis using flow cytometry
Flow cytometry allows cells from any organism, tissue or cell culture to be analysed. Cells in suspension can be labelled with antibodies or fluorescent probes, then go through lasers that measures their size, complexity and fluorescence. This approach provides detailed information about each cell, quickly and accurately.
Flow cytometry has several applications:
- Morphological analysis
- Phenotyping (population abundance or protein expression)
- DNA content
- Cell cycle and proliferation
- Cell viability
- Mitochondrial membrane potential
- Cytokine multiplexing
- Calcium flux
- Cell sorting
- …
Expertise in flow cytometry
Our Personalized Support includes :
- Experimental Design: Support in defining objectives and choosing the most appropriate controls for your experiment.
- Protocol Design: Expert advice on sample preparation, ensuring optimal data acquisition or high-quality cell sorting.
- Panel Design: Assistance in selecting the most relevant fluorochrome combinations
- Data acquisition: Complete execution by our teams or personalised assistance for you to carry out your own acquisitions independently
- Data Analysis: Processing of data, visualisation and interpretation of results according to your experimental needs.
- Cell Sorting: Performed by our experienced staff to ensure reliable and reproductible results
- Training: Development of your skills through practical training in the independent use of our instruments and analysis software.
Technical description of our equipments
Our flow cytometry core facility is equiped with 2 analysers (BD FACSVerse and Cytoflex S) and 1 cell sorter (Cytoflex SRT).
Analysers
BDFACS Verse
- 3 lasers (405nm, 488nm, 633nm)
- 8 colors + FSC/SSC parameters
- Sample in FACS tube or microplates
- 48 or 52 places rack autonomous sampler
Cytoflex S
- 4 lasers (405nm, 488nm, 561nm, 638nm)
- 13 colors + FSC/SSC parameters
- Sample in FACS tube ; eppendorf or microplates
- Microplates autonomous sampler
Sorters
Cytoflex SRT
- 4 lasers (405nm, 488nm, 561nm, 638nm)
- 15 colors + FSC/SSC parameters
- Sample in FACS tube; eppendorf or falcon 15
- 4-way (eppendorf, FACS tube, falcon 15) and microplate sorting
Analysis software
CytExpert
FlowJo
Contacts
- Céline Legrand | Research Logistician
celine.legrand@unamur.be - Kévin Willemart | Technician
kevin.willemart@unamur.be - Jean-Yves Matroule | Academic Manager
jean-yves.matroule@unamur.be