At the end of this course, the student must:
Know how viruses are structured
Understand how viruses enter the cell
Understand how viruses replicate in the cell
Understand what the cell can do to limit viral replication
Understand how viruses exit the cell?
Understand how viruses evolve
Understand how to cultivate viruses
Understand how to identify individuals infected by a virus
Understand how to prevent viral replication in vivo
Understanding the structure of viruses
Understanding how viruses enter cells
Understanding how viruses replicate within cells
Understanding what cells can do to limit viral replication
Understanding how viruses leave cells
Understanding how viruses evolve
Understanding how to culture viruses
Understanding how to identify individuals infected with a virus
Understanding how to prevent viral replication in vivo
The course will describe the viral cycle, from the entry of the virus into the host organism to the infection of a new individual, including the hijacking of the cellular machinery and the subversion of the immune response. The different taxa will be detailed, in particular during the presentation of the mechanisms of replication of the viral genome. Finally, different antiviral strategies will be presented.
Introduction to virology
Virus culture and identification
Viral genome and genetics
Capsid and envelope structure
Virus attachment and entry
Viral RNA synthesis from an RNA model
Viral RNA transcription and maturation
DNA virus genome replication
Reverse transcriptase and integrase
Translation
Virion assembly, exit and maturation
Virus evolution
Antivirals
Ex cathedra course with PowerPoint presentations.
Short video covering key concepts.
Written exam covering the material seen in theoretical courses and given during the practical work.
Principles of Virology, 4th edition, Flint, Racaniello, Rall and Skalka, ASM press, ISBN 978-1-55581-9514
ViralZone, http://viralzone.expasy.org/